From BLAST alignments to answers — in minutes.
Every molecular diagnostics lab runs NCBI BLAST. None of them get an answer — just raw data. Until now.
Watch BLASTseq AI run a full analysis
From sequence input to analysis reports in minutes — see the complete in silico workflow.
BLASTseq AI demo · 2 min
Everything your in silico workflow needs, automated by AI
From BLAST alignments to shareable reports — BLASTseq AI replaces days of manual bioinformatics work.
Supercharge In-Silico Analysis & Assay Performance
- Automate Surveillance: Streamline post-market reporting for DNA/RNA assays.
- Accelerate Analysis: Complete BLAST alignments and variant checks in minutes.
- Optimize Design: Run smart in-silico inclusivity and exclusivity screening.
- Predict Performance: Accurately forecast primer/probe binding for qPCR.
Automate Primer-Probe Verification & Surveillance
- Inclusivity & Exclusivity: Ensure your assay detects the exact target of interest while automatically excluding cross-reactive species to prevent false positives.
- Precise Alignment Data: Instantly view oligonucleotide binding locations, homology data, and amplifiability across all accessions.
- Optimize Wet Testing: Accelerate laboratory work with AI-calculated overall detection rates based on precise primer-probe binding regions.
Accurate Variant Calling & Annotation
- Pinpoint Anomalies: Confidently identify genetic variations, mutations, and sequence anomalies using advanced, highly accurate algorithms.
- NCBI Database Integration: Automatically detect mutation and variant sequences for your specific amplicon or target within the NCBI BLAST database.
- Streamlined Grouping: Accelerate your workflow with results automatically grouped by identical sequences, so you can instantly identify and download relevant accessions.
- Detailed Performance Checks: Generate comprehensive, automated reports to verify assay performance and confirm inclusivity/exclusivity parameters.
AI Summary Reports & Data Visualization
- Intelligent Data Parsing: Instantly sort and separate your results into “detected” and “not detected” accession groups for rapid analysis.
- Automated Reporting: Generate customizable, comprehensive reports detailing inclusivity, exclusivity, and mutation/variant analysis with zero manual effort.
- Actionable Clarity: Transform dense genomic data into clear, team-ready summaries that highlight your most critical findings.
- Share Analysis: Instantly share your analysis and reports with other users.
See exactly what BLASTseq AI produces
Every run delivers structured, interrogable output — from raw alignments to detection groups, inclusivity/exclusivity summaries, and variant calls.
A detailed tabulation of BLAST search results — a comprehensive overview of identified sequences and their characteristics across all matched accessions.
| Sr | Description | Query cover | E-value | Identity | Accession |
|---|---|---|---|---|---|
| 1 | Acanthamoeba sp. isolate AcW110A small sub… | 100% | 9.9e-85 | 100% | MZ272079 |
| 2 | Acanthamoeba sp. isolate LC6M4 small subu… | 100% | 9.9e-85 | 100% | PV810227 |
| 3 | Acanthamoeba sp. isolate ZWR-16-Q2-D 18S… | 100% | 9.9e-85 | 100% | MK390870 |
| 4 | Acanthamoeba sp. AcL-BL11 partial 18S rRNA… | 100% | 9.9e-85 | 99.6% | HF930495 |
| 5 | Acanthamoeba sp. AC6 gene for 18S rRNA, p… | 100% | 9.9e-85 | 99.6% | AB554223 |
| 6 | Acanthamoeba genotype T4 small subunit rib… | 100% | 9.9e-85 | 100% | OR364831 |
| 7 | Acanthamoeba genotype T4 isolate Chon3 18… | 100% | 9.9e-85 | 100% | KF733235 |
Detailed sequence alignments showing how the query aligns against each matched accession, with mismatches highlighted base-by-base.
Oligonucleotide binding locations and PCR amplifiability per accession.
| Accession | Fwd primer | Probe | Rev primer | Amplifiable |
|---|---|---|---|---|
| MZ272079 | bind | bind | bind | Yes |
| PV810227 | bind | 1 mm | bind | Yes |
| MK390870 | bind | bind | bind | Yes |
| HF930495 | 2 mm | bind | bind | Partial |
| AB554223 | bind | bind | no | No |
| OR364831 | bind | bind | bind | Yes |
Accessions categorised by detection status — instantly assess the outcome of organism detection across your full result set.
In-silico inclusivity & exclusivity summary — confirm your assay detects the target while excluding cross-reactive species.
| Target organism | Accessions | Detected | Inclusivity | |
|---|---|---|---|---|
| Acanthamoeba T4 | 842 | 840 | 99.8% | |
| Acanthamoeba T3 | 214 | 212 | 99.1% | |
| Acanthamoeba T5 | 156 | 154 | 98.7% | |
| Acanthamoeba T11 | 80 | 78 | 97.5% | |
| Off-target spp. | 3,401 | 0 | excluded |
Assessment of insertions, deletions and mismatches, and their impact on assay specificity and target detection.
| Position | Type | Change | Frequency | Impact |
|---|---|---|---|---|
| probe +12 | SNP | G → A | 2.1% | Reduced |
| fwd +4 | SNP | C → T | 0.6% | None |
| rev +18 | Deletion | −AG | 0.2% | Detection loss |
| amp +71 | SNP | T → C | 4.8% | None |
| amp +96 | Insertion | +T | 0.1% | None |
Three steps from sequence to signed-off report
Paste your sequence
Drop in your primer, probe or amplicon sequence — or an existing assay design. No file wrangling, no setup.
AI runs BLAST & filters
BLASTseq aligns against the live NCBI database, applies taxonomy-based filtering, and groups identical hits automatically.
Get the report
Inclusivity, exclusivity and variant findings in a clean, shareable report — ready for your team or submission file.
Used by scientists at molecular diagnostics labs during early access.
“I've been manually interpreting BLAST results for years. BlastSeq.AI gave me the same answer in under two minutes. I didn't think that was possible.”
“The inclusivity screening alone saves me an entire day of work per assay. This should have existed a long time ago.”
“What I appreciated most was that it didn't just give me a score — it explained the result. That's what we actually need when writing up validation reports.”
“We run BLAST on every primer and probe we design. BlastSeq.AI has completely changed how fast we can move from sequence design to validation decision.”
Plans that scale from student to enterprise
Start free and pay as you go, or subscribe for a monthly credit allowance. Pricing is per user — 1 credit per AI prompt, 200 credits to unlock a full report.
- Dashboard analytics
- AI assistant access (credits)
- Unlock reports with credits
- Share analysis runs
- Monthly subscription credits
- Everything in Basic
- 200 monthly credits included
- Download grouped variant reports
- Buy additional credits anytime
- Chat & email support
- Everything in Pro
- 400 monthly credits included
- Share runs & reports across org
- Higher credit allocation
- Chat, email & phone support
🎁 20 welcome credits on signup — plus an extra 200 bonus credits on your first purchase of 20+ credits.
Questions & answers
Everything you need to know about how BLASTseq AI fits your workflow.
Browse all FAQsSupercharge your BLAST analysis & assay-performance with AI
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